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A Dual Selection Module for Directed Evolution of Genetic Circuits

Authors:	Yohei?Yokobayashi,Frances?H.?Arnold author-information" > author-information__contact u-icon-before" > mailto:frances@cheme.caltech.edu" title=" frances@cheme.caltech.edu" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author

Affiliation:	(1) Division of Chemistry and Chemical Engineering, California Institute of Technology, mail code 210-41, Pasadena, California 91125, USA;(2) Present address: Department of Biomedical Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA

Abstract:	We have developed a genetic selection module which enables us to select for positive and negative expression of a gene by controlling the expression of two antibiotic resistance genes. This module can be used to tune the performance of genetic circuits that yield simple ON/OFF gene expression outputs depending on the environment (input). We have demonstrated the utility of the module by selecting a genetic inverter from a 200 fold excess of nonfunctional inverters in two rounds.

Keywords:	cellular computation directed evolution genetic circuits selection synthetic biology synthetic gene circuits
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