| N+和Ti+离子注入井冈霉素生产菌诱变筛选高产菌株的研究 |
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| 引用本文： | 虞龙,安肖.N+和Ti+离子注入井冈霉素生产菌诱变筛选高产菌株的研究[J].辐射研究与辐射工艺学报,2007,25(6):371-374. |
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| 作者姓名： | 虞龙 安肖 |
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| 作者单位： | 南京工业大学制药与生命科学学院,南京,210009 |
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| 摘 要： | 利用N 和Ti 离子注入对井冈霉素生产菌株进行诱变选育,比较了出发菌株经过两种离子源注入后的诱变效应.初步探索使用金属离子和气体离子交替注入诱变方法,得到了较高的正突变率和突变增幅.用该方法诱变得到一株高产菌株B1-3,其产井冈霉素A组分的效价为21514,比出发菌株提高54.4%.经多次传代实验表明该菌株的遗传稳定性较好.
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| 关 键 词： | 离子注入 诱变 选育 井冈霉素 |
| 收稿时间： | 2007-04-11 |
| 修稿时间： | 2007-07-12 |
Screening on the high yield validamycin producing strain by implantation with N+ and Ti+ ion sources |
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| YU Long,AN Xiao.Screening on the high yield validamycin producing strain by implantation with N+ and Ti+ ion sources[J].Journal of Radiation Research and Radiation Processing,2007,25(6):371-374. |
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| Authors: | YU Long AN Xiao |
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| Abstract: | In order to compared the mutagenic effects of the validamycin producing the swain(Streptomyces hy- groscupicus var.Jingganggensis Yen.) was implanted with two kinds of ion sources.The results showed that when two kinds of ion sources implanted into the strain by turns,more positive mutants and higher yield would be acquired. Using this method,a high-yielding strain B1-3 was obtained,which produce the titer of validamycin A of 21514,and was 54.4% higher than that of the original swain. |
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| Keywords: | Ion implantation Mutation Validamycin Jinggangmycin |
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