液质联用法测定淀粉中的马来酸

采用HPLC-ESI-MS/MS联用技术,检测淀粉及其制品中马来酸残留量。样品经50%水-50%甲醇提取后,以C18柱为液相分离柱,以甲醇和1%甲酸为流动相,多反应监测模式测定马来酸,其定性定量离子对分别为114.9/71.0。马来酸含量在10~100ng/mL范围内具有良好的线性关系,测定下限(10S/N)为10ng/mL。以淀粉为基体,加入3种不同浓度水平的马来酸做加标回收试验,回收率在85%~100%,相对标准偏差(n=6)小于10%。该方法操作简单,结果准确,重现性好。

Determination of Starch Maleate by LC-MS/MS

Using HPLC-ESI-MS/MS hyphenaled techniques, starch and its products were deteeted residues of maleie aeid. Sample was 50% water and 50% methanol extract in the liquid phase C18 cohmm separalion column with methanol and 1% formie aeid as the mobile phase, multiple reaetion monitoring mode determination nmleie acid, qualitative and quantitative ion pairs were 114.9/71.0. Maleie acid had a good linear relationship within the 10-100ng/mL , detection limit （ IOS/N ） was 10ng/mL. Starch matrix, adding three different concentration levels did maleate spiked reeovery test, recovery reached between 85% to 100% and relative standard deviation （n=6） of less than 10%. This method was simple, accurate, and reproducible.