| 蓖麻毒蛋白分离条件优化 |
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| 引用本文： | 李楠,黎继烈,刘汝宽,吴红.蓖麻毒蛋白分离条件优化[J].粮油加工,2014(4):49-52. |
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| 作者姓名： | 李楠 黎继烈 刘汝宽 吴红 |
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| 作者单位： | [1]中南林业科技大学经济林培育与保护省部共建教育部重点实验室,湖南长沙410004 [2]湖南省林业科学院,湖南长沙410004 |
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| 基金项目： | 国家科技支撑计划项目（2011BAD22B04）;生物学湖南省重点学科经费资助 |
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| 摘 要： | 本文以蓖麻蛋白为原料,通过单因素试验和正交试验优化对毒蛋白纯化工艺进行优化。结果表明:最佳工艺条件为缓冲液pH值7.0、缓冲液浓度0.10mol/L、D-半乳糖浓度0.10mol/L和洗脱速度1.0mL/min。优选所得工艺设计合理、结果可靠,可作为蓖麻毒蛋白的分离纯化工艺。
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| 关 键 词： | 蓖麻毒蛋白 亲和层析 分离纯化 |
Optimized Conditions for Ricin Extraction Process |
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| Li Nan,LiJilie,Liu Rukuan,Wu Hong.Optimized Conditions for Ricin Extraction Process[J].Cereals and Oils Processing,2014(4):49-52. |
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| Authors: | Li Nan LiJilie Liu Rukuan Wu Hong |
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| Affiliation: | 1. Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees Ministry of Education Central South University of Forestry Science And Technology, Changsha City, Hunan Province 410004 ;2. Hunan Province Forestry Research Institute, Changsha City, Hunan Province 410004) |
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| Abstract: | Ricin was extracted from crude castor protein. Single-factor experimenls and orthogonal design of experiments were used to optimize and the optimal condition were as followed ： buffer pH 7.0, Duffer concentration 0.10mol/ L, D-galaetose concentration 0.10mol/L, elution speed 1.0mL/min. Optimal design reasonable and reliable, it can be used as separation purification process of ricin. |
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| Keywords: | Ricin Affinity chromatography Separatiou and purification |
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