Extraction of pomegranate (Punica granatum L.) seed oil using superheated hexane

Superheated hexane extraction (SHHE), Soxhlet extraction and cold pressing method were compared for the extraction of pomegranate seed oil. The extraction efficiencies of different temperatures (80, 100, 120 °C), mean particle sizes (0.25, 0.50 and 1.00 mm), and n-hexane flow rates (0.5, 1.0 and 2.0 mL/min) were investigated. The fatty acids profile of the seed oils were quantitatively determined by GC-FID. The optimum extraction temperature, mean particle size, and flow rate were selected as 80 °C, 0.25 mm, and 1 mL/min, respectively. The SHHE showed a higher extraction efficiency (22.18 wt%) within 2 h than Soxhlet extraction (17.94 wt%) for 24 h and cold pressing (4.29 wt%) for 72 h. The fatty acids profile for SHHE was more similar to that obtained by cold pressing method than Soxhlet extraction.