| 同位素示踪法测定~(125)I-NGF在小鼠体内的血药浓度变化 |
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| 引用本文： | 姜国华,黄建华,刘忠敏. 同位素示踪法测定~(125)I-NGF在小鼠体内的血药浓度变化[J]. 现代仪器, 2007, 13(2): 9-10 |
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| 作者姓名： | 姜国华 黄建华 刘忠敏 |
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| 作者单位： | 北京师范大学分析测试中心,北京,100875 |
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| 摘 要： | 本文涉及应用Idogn法对神经生长因子(NGF)进行125I标记并利用同位素示踪与电泳分离相结合的方法测定125I-NGF在小鼠体内的血药浓度与时间变化关系。结果显示,静脉注射125I-NGF在小鼠体内的代谢规律符合二房室开放模型。分布相半衰期为0.13h,消除相半衰期为3.68h,125I-NGF在小鼠体内分布和消除均较快,清除率为0.125L/h/Kg,表观分布容积为0.697L/kg,曲线所围面积为16.01μg.h/L。
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| 关 键 词： | 神经生长因子 125I标记 同位素示踪法 十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 |
Determination of the change of 125I-NGF concentration in plasma in mouse by isotope tracing method |
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| Jiang Guohua,Huang Jianhua,Liu Zhongmin. Determination of the change of 125I-NGF concentration in plasma in mouse by isotope tracing method[J]. Modern Instruments, 2007, 13(2): 9-10 |
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| Authors: | Jiang Guohua Huang Jianhua Liu Zhongmin |
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| Affiliation: | Analytical and Testing Centre Beijing Normal University Beijing 100875 |
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| Abstract: | The study concerns in the label of nerve growth factor(NGF)by 125I with Idogn method.The change of 125I-NGF Concentration in Plasma in Mouse with time was determinated using the method of sodium dodecylsilfatepoly-accrylamide gel electrophoresis(SDS PAGE)after iv 125I-NGF.Result demonstrate the time-125I-NGF concentration in plasma curve after iv fit a two compartment open model.Distribution phase half life is 0.13h,elimination phase half life is 3.68h,the mean plasma clearance is 0.125L/(h·Kg),apparent volume of distribution is 0.697L/kg,area under curve is 16.012μg·h/L. |
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| Keywords: | Nerve growth factor(NGF) Isotope tracing method SDS PAGE |
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