纺织品中双酚 A 检测用表面等离子体共振传感器的表面预处理

针对纺织服装中双酚 A（BPA）分子量小，直接采用表面等离子体共振（SPR）技术难以精准检测的问题，采用传感器表面预处理方法和溶液竞争原理来扩大响应信号，对其进行定性定量检测。为更好地使双酚Ａ?牛血清白蛋白抗原（BPA?BSA）和传感芯片进行氨基偶联，从传感芯片的选择、修饰溶液的pH 值、离子浓度、BPA?BSA 抗原的最佳浓度４ 个因素出发，运用控制变量法，探索双酚Ａ 检测中传感芯片的最佳修饰条件。结果表明：当采用羧甲基葡聚糖组装（CM5） 芯片、修饰液pH 值为4.5、离子浓度为10 mmol/L、BPA?BSA 质量浓度为50 μg/mL 时，BPA?BSA 的氨基偶联修饰效果最佳，且预处理方法简便快捷，BPA 无需精度提纯，特异性识别度高，可应用在纺织品双酚 A 检测中。

Surface pretreatment method for surface plasmon resonance sensor fordetecting bisphenol A in textiles

The bisphenol A in textiles and clothing has low molecular weight, that is kifficult to be accurately measured. The surface plasmon resonance (SPR) and solution competition method was used to detect it. In order to better bind bisphenol A and bovine serum albumin antigens (BPA-BSA) with sensing chips to generateamino groups, four factors including selection of the sensing chip, the modification of pH for thesolutions, the ion concentration and the optimal concentration ofBPA-BSA were taken into account. By controlling the variates of the four factors, the best condition for sensor chip to detect BPA was edtermined. The results show that the best amino-coupled effect of BPA-BSA occurs using carboxymethyl dextran assembly (CM5) chip atpH 4.5, the ion concentrationof 10 mmol/L and the concentration of BPA-BSA of 50μg / mL. The pretreatment method is simple and efficient, and high specificity BPA an detected without precision purification. This method can be used in textiles for BPA detection.