壳聚糖基纳米纤维载药体系及其缓释行为

为研制具有缓释效果的抗菌材料，以壳聚糖(CS)、聚乙烯醇(PVA)为原料，采用静电纺丝技术制备CS/PVA 复合纳米纤维膜并负载环丙沙星；探究纺丝体系、纺丝工艺对纤维膜微观形貌、接触角、化学结构的影响，分析药物体外释放行为及载药前后试样的抗菌性。结果表明：PVA 的加入提高了CS 的可纺性；改善了纤维膜的亲水性；当纺丝电压为24 kV、CS 和PVA 质量比为1：1. 5 时，纤维膜成网均匀，形貌良好；载药纳米纤维膜具有相对较低的药物释放速率，可有效避免药物突释，且药物释放速率随纤维膜中环丙沙星质量分数的增大而增大；载药CS/PVA 纳米纤维膜对金黄色葡萄球菌具有优良的抗菌性。

Chitosan based nanofiber drug loaded system and its sustained release behavior

To develop antibacterial materials with sustained release effect, chitosan (CS), polyvinyl alcohol (PVA) and ciprofloxacin were used to prepare a drug loaded CS/ PVA composite nanofiber membrane by electrospinning. The influence of spinning system and process parameters on the micromorphology, contact angle and chemical structures of the nanofiber membrane were explored, as well as the in vitro drug delivery behavior and antibacterial capability before and after drug loading. The results indicate that the addition of PVA enhances the spinnability of CS and the hydrophilicity of the nanofiber membrane. Nanofiber membranes with high uniformity and good modality are obtained when the applied voltage is 24 kV and the mass ratio of CS and PVA is 1 ∶ 1. 5. The drug loaded CS/ PVA composite nanofiber membranes exhibit a relative low release rate, which can effectively avoid burst release. In addition, the rate of drug release rises with the increase of ciprofloxacin concentration in nanofiber membranes. The results of antibacterial experiment show that drug loaded CS/ PVA nanofiber membrane has excellent antibacterial activity against staphylococcus aureus.