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诺丽酵素抗氧化性能与蛋白质营养价值评价研究
引用本文:陈小伟,程勇杰,薛淑龙,方晟,张婷,崔艳丽,毛建卫,沙如意. 诺丽酵素抗氧化性能与蛋白质营养价值评价研究[J]. 中国酿造, 2018, 37(9): 115. DOI: 10.11882/j.issn.0254-5071.2018.09.023
作者姓名:陈小伟  程勇杰  薛淑龙  方晟  张婷  崔艳丽  毛建卫  沙如意
作者单位:1.浙江科技学院 生物与化学工程学院 浙江省农产品化学与生物加工技术重点实验室 浙江省农业生物资源生化制造协同 创新中心,浙江 杭州 310023;2.绍兴文理学院 元培学院,浙江 绍兴 312000;3.浙江大学 化学系,浙江 杭州 310027
基金项目:浙江省重点研发计划项目(2017C02009);中国博士后科学基金(2018M632475);浙江省农业生物资源生化制造协同创新中心开放基金项目(2016KF0040;2016KF0114)
摘    要:对3种不同发酵时间的诺丽酵素体外抗氧化能力、氨基酸的含量以及蛋白质营养评价进行研究,结果表明:随着发酵时间的 延长,诺丽酵素中总酚含量有轻微上升;3种酵素均具有抗氧化能力,且均表现出显著的浓度依赖性(P<0.05),发酵时间长短对其影 响不明显(P>0.05);随着发酵时间的延长,蛋白质含量有轻微下降,3种酵素均检测出16种常见蛋白氨基酸和γ-氨基丁酸(GABA), 半胱氨酸(Cys)未检出,GABA含量随着发酵时间的延长有所上升,3种酵素中必需氨基酸含量丰富;9种必需氨基酸得分(AAS)均> 100%,比值系数分(SRC)均>55,单从蛋白质营养价值来说,从高到低依次为:发酵360 d诺丽酵素>发酵540 d诺丽酵素>发酵720 d 诺丽酵素。

关 键 词:诺丽  酵素  抗氧化  氨基酸  营养价值评价  

Evaluation of antioxidant activity and nutritional value of protein in noni Jiaosu
CHEN Xiaowei,CHENG Yongjie,XUE Shulong,FANG Sheng,ZHANG Ting,CUI Yanli,MAO Jianwei,SHA Ruyi. Evaluation of antioxidant activity and nutritional value of protein in noni Jiaosu[J]. China Brewing, 2018, 37(9): 115. DOI: 10.11882/j.issn.0254-5071.2018.09.023
Authors:CHEN Xiaowei  CHENG Yongjie  XUE Shulong  FANG Sheng  ZHANG Ting  CUI Yanli  MAO Jianwei  SHA Ruyi
Affiliation:1.Zhejiang Provincial Collaborative Innovation Biochemical Manufacturing, Center of Agricultural Biological Resources, Zhejiang Provincial key lab for Chem & Bio Processing Technology of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science & Technology, Hangzhou 310023, China; 2.Yuanpei College of Shaoxing University, Shaoxing 312000, China; 3.Department of Chemistry, Zhejiang University, Hangzhou 310027, China
Abstract:The in vitro antioxidant activity, evaluation of amino acids content and protein nutrition of noni Jiaosu in three kinds of fermentation time were discussed in this paper. The results showed that with the prolongation of fermentation time, the content of total phenols in noni Jiaosu increased slightly, and the three kinds of Jiaosu had antioxidant activity, with certain concentration dependence (P<0.05), and the fermentation time had no obvious effect on the antioxidant activity (P>0.05). The protein content decreased slightly with the prolongation of fermentation. Except for Cys, 16 common protein amino acids and non-protein amino acids GABA were all detected in three kinds of Jiaosu, and the GABA content increased to some extent along with the fermentation time. The essential amino acids were rich in three noni Jiaosu, and the amino acid score (AAS) of nine essen- tial amino acids were greater than 100%, meanwhile the score of ratio coefficient of amino acid/essential were greater than 55. The nutritional value of protein from high to low were as follows: noni Jiaosu fermented for 360 d>540 d>720 d.
Keywords:noni  Jiaosu  antioxidant  amino acid  nutritional value evaluation  
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