| 一株刺盘孢属菌的分类鉴定及转化孕酮和雄烯二酮的研究 |
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| 引用本文： | 张广求,段焰青,马慧宇,刘赟,杜刚.一株刺盘孢属菌的分类鉴定及转化孕酮和雄烯二酮的研究[J].中国酿造,2018,37(5):103. |
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| 作者姓名： | 张广求 段焰青 马慧宇 刘赟 杜刚 |
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| 作者单位： | （1.云南民族大学 电气信息工程学院 云南 昆明 650500；2.云南中烟工业有限责任公司技术中心，云南 昆明 650202； 3.云南烟草质量监督检测站，云南 昆明 650106；4.云南民族大学 民族药资源化学国家民族事务委员会教育部重点实验室， 云南 昆明 650500） |
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| 基金项目： | 云南省教育厅科学研究基金重点项目（2015Z111）；云南省技术创新人才项目（2016HB009） |
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| 摘 要： | 研究了菌株YNCA0116对孕酮和4-雄烯二酮（4-AD）的生物转化，并对该转化菌株进行形态学和分子生物学鉴定，转化产物经分离纯化后，运用核磁共振（NMR）、质谱（MS）、红外光谱（IR）分析进行结构鉴定。结果鉴定，该菌株为刺盘孢属（Colletotrichum sp.），该菌转化孕酮得到主产物11α,15α-二羟基孕酮和副产物6β,11α-二羟基孕酮，转化4-AD得到11β,15α-二羟基-4-雄烯二酮。刺盘孢属菌株YNCA0116具有转化甾体二羟基化的能力，对不同甾体化合物羟基化特点有差异。
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| 关 键 词： | 微生物转化 刺盘孢属菌株 羟基化 甾体化合物 |
Taxonomic identification and transformation of progesterone and 4-androstenedione by a strain of Colletotrichum sp. |
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| ZHANG Guangqiu,DUAN Yanqing,MA Huiyu,LIU Yun,DU Gang.Taxonomic identification and transformation of progesterone and 4-androstenedione by a strain of Colletotrichum sp.[J].China Brewing,2018,37(5):103. |
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| Authors: | ZHANG Guangqiu DUAN Yanqing MA Huiyu LIU Yun DU Gang |
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| Affiliation: | (1.School of Electrical and Information Technology, Yunnan Minzu University, Kunming 650500, China; 2.Technology Center, China Tobacco Yunnan Industrial Limited Company, Kunming, 650202, China; 3.Yunnan Tobacco Quality Supervision and Test Station, Kunming 650106, China; 4.Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education, Yunnan Minzu University, Kunming 650500, China) |
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| Abstract: | The transformation of progesterone and 4-androstenedione (4-AD) by strain YNCA0116 were studied, and the strain was identified according to its morphological characters and molecular biological analysis. The transformation products were separated and their structures were elucidated with spectroscopic methods including NMR, MS and IR. Results showed that the strain was Colletotrichum sp., the principal products and by-products of progesterone converted by the strain were 11α,15α-dihydroxyprogesterone and 6β,11α-dihydroxyprogesterone. and the converted product of 4-AD was 11β,15α-hydroxy-4-andronstenedione. The results showed that the steroids could be converted and dihydroxylized by Colletotrichum sp. YNCA0116, and the hydroxylation characteristics were different in different steroids. |
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| Keywords: | microbial transformation Colletotrichum sp hydroxylation steroids |
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