响应面法优化无花果叶总黄酮超声辅助提取工艺

采用超声波辅助提取无花果叶中总黄酮的工艺，并考察了其抗氧化活性。根据单因素试验结果，用响应面法对总黄酮提取工艺进行优化。结果表明，最佳提取工艺条件为：乙醇体积分数70%，液料比30∶1（mL∶g），超声时间25 min，超声温度70 ℃。此最佳条件下，总黄酮提取率为27.379 mg/g，而模型预测总黄酮提取率为27.384 mg/g，理论预测值与试验结果接近。通过试验得出，该提取物对ABTS自由基、超氧阴离子自由基清除率分别为65.23%、73.21%，证明其具有较好的体外抗氧化能力。

Optimization of ultrasonic-assisted extraction process of total flavonoids from Ficus carica leaves

Ultrasonic-assisted total flavonoids extraction from Ficus carica leaves and the antioxidant activity were studied in the paper. According to single factor experimental results, the ultrasonic-assisted extraction process was optimized using response surface methodology. The results showed that the optimal extraction process was ethanol concentration 70%, liquid-solid ratio 30∶1 (ml∶g), ultrasound time 25 min, temperature 70 ℃. Under the optimal conditions, the extraction rate of total flavonoids was 27.379 mg/g and it was close with the predicted value from the theoretical model which was 27.384 mg/g. Antioxidant determination of extracts was carried out. The results showed that the removal rate of ABTS radical and superoxide anion radical were 65.23% and 73.21%, respectively, which had good in vitro antioxidant capacity.