基于Miseq高通量测序技术的古襄阳酒窖泥细菌多样性评价

使用Miseq高通量测序技术对4份古襄阳酒业浓香型白酒窖泥样品的细菌多样性进行了评价，同时通过选择性培养分离出窖 泥中的优势乳酸菌，再利用16S rDNA序列分析方法，对其进行初步鉴定。 结果表明，乳酸杆菌是古襄阳酒窖池中的优势细菌，其相对 含量高达92.02%。在分类操作单元（OTU）水平上，发现8个核心OTU相对含量＞1.0%，其中7个隶属于乳酸杆菌属（Lactobacillus）。4份 古襄阳酒业浓香型白酒窖泥样品中共分离到12株菌株，初步鉴定其全部为副干酪乳杆菌（Lactobacillus paracasei）。

Bacterial diversity in pit mud of Guxiangyang Baijiu analyzed by Miseq high-throughput sequencing

To investigate the diversity of bacterial microflora in pit mud of Guxiangyang Luzhou-flavor Baijiu, 4 samples were collected and the bacte- rial microflora was studied by Miseq high-throughput sequencing technology. Meanwhile, the dominate lactic acid bacteria were isolated from pit mud samples by selective culture method, and preliminary identified by 16S rDNA sequencing. Results showed that Lactobacillus was the dominant bacteria in pit mud of Luzhou-flavor Baijiu with the relative abundance of 92.02%. At the OTU level, it showed that 8 core OTUs had the relative abundance of higher than 1.0%, and 7 OTUs were belonged to Lactobacillus. The results also showed that 12 strains of lactic acid bacteria were iso- lated from 4 pit mud samples of Guxiangyang Luzhou-flavor Baijiu, and they were all identified as Lactobacillus paracasei.