Identification of drug metabolites in biological matrices by intelligent automated liquid chromatography/tandem mass spectrometry |
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Authors: | LL Lopez X Yu D Cui MR Davis |
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Affiliation: | ThermoQuest Finnigan, San Jose, CA 95134, USA. |
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Abstract: | A rapid and systematic strategy for the identification of drug metabolites in biological matrices based on liquid chromatography-tandem mass spectrometry (LC/MS/MS) techniques was utilized for the identification of drug metabolites of the HIV protease inhibitor Indinavir. This strategy integrates intelligent realtime mass spectrometry with HPLC detection and a predictive strategy for detecting metabolites arising from common biotransformations, to rapidly elucidate structures of drug metabolites. Structures of metabolites generated from in vitro incubation mixtures of Indinavir were characterized from a single chromatographic analysis using the automated LC/MS/MS methodology, thus reducing data acquisition time and improving efficiency. |
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