Combined in vivo multiphoton and CARS imaging of healthy and disease-affected human skin |
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Authors: | Breunig Hans Georg Bückle Rainer Kellner-Höfer Marcel Weinigel Martin Lademann Jürgen Sterry Wolfram König Karsten |
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Affiliation: | JenLab GmbH, Campus D1.2, 66123 Saarbrücken, and Schillerstr 1, 07745, Jena, Germany. breunig@jenlab.de |
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Abstract: | We present combined epi-coherent anti-Stokes Raman scattering (CARS) and multiphoton imaging with both chemical discrimination and subcellular resolution on human skin in vivo. The combination of both image modalities enables label-free imaging of the autofluorescence of endogenous fluorophores by two-photon excited fluorescence, as well as imaging of the distribution of intercellular lipids, topically applied substances and water by CARS. As an example for medical imaging, we investigated healthy and psoriasis-affected human skin with both image modalities in vivo and found indications for different lipid distributions on the cellular level. |
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Keywords: | CARS tomography non‐linear microscopy skin imaging multiphoton imaging |
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