| 响应面法优化麦胚凝集素分离纯化工艺研究 |
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| 引用本文： | 吴定,刘长鹏,路桂红,张丽莉,袁少华,张伟华.响应面法优化麦胚凝集素分离纯化工艺研究[J].中国粮油学报,2011,26(11):23-26,31. |
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| 作者姓名： | 吴定 刘长鹏 路桂红 张丽莉 袁少华 张伟华 |
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| 作者单位： | 南京财经大学食品科学与工程学院,南京,210003 |
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| 基金项目： | 江苏省教育厅高新技术产业 |
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| 摘 要： | 麦胚凝集素是一种具有生物学活性的蛋白质,将其从麦胚中提取、开发,能促进麦胚的综合利用,提高相关生产企业经济效益。依据Box-Behnken试验设计原理,进行饱和度硫酸铵质量分数、甲壳素用量和甲壳素吸附麦胚凝集素时间的响应面优化分析。建立了提取、纯化麦胚凝集素优化工艺的二次多项数学模型。优化麦胚凝集素分离、纯化工艺参数:饱和硫酸铵质量分数为47.5%、甲壳素用量为2.0:1和甲壳素吸附麦胚时间为19 h。优化工艺模型预测麦胚凝集素相对得率的理论值为92.1%,验证试验显示麦胚凝集素实际提取率为92.7%。
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| 关 键 词： | 麦胚凝集素 甲壳素 分离与纯化 响应面分析法 |
Study on Optimal Technique on Isolation and Purification of Wheat Germ Agglutinin in Response Surface Methodology |
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| Wu Ding,Liu Chengpen,Lu Guihong,Zhang Lili,Yu Saohua,Zhang Weihua.Study on Optimal Technique on Isolation and Purification of Wheat Germ Agglutinin in Response Surface Methodology[J].Journal of the Chinese Cereals and Oils Association,2011,26(11):23-26,31. |
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| Authors: | Wu Ding Liu Chengpen Lu Guihong Zhang Lili Yu Saohua Zhang Weihua |
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| Affiliation: | Wu Ding Liu Chengpen Lu Guihong Zhang Lili Yu Saohua Zhang Weihua (School of Food Science and Engineering,Nanjing University of Finance and Economics,Nanjing 210003) |
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| Abstract: | Wheat germ agglutinin is a kind of protein with a biological activity and a wheat flour production factory can enhance economical value when wheat germ agglutinin are extracted from wheat germ and used in many domain of science.On principle of Box-Behnken experiment design,the saturated ammonium sulfate mass fraction,chitosan mass fraction and absorption time of chitosan for wheat germ agglutinin were optimally analyzed with the Response Surface Methodology.The mathematical model of quadratic polynomial on ... |
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| Keywords: | wheat germ agglutinin chitosan isolation and purification response surface methodology |
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