| 基于负二阶导数红外光谱的反式脂肪酸的快速测定 |
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| 引用本文： | 陶健,丁太春,冯波,蒋炜丽,杨智灵,苏亮. 基于负二阶导数红外光谱的反式脂肪酸的快速测定[J]. 中国粮油学报, 2011, 26(11): 115-118 |
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| 作者姓名： | 陶健 丁太春 冯波 蒋炜丽 杨智灵 苏亮 |
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| 作者单位： | 河南省产品质量监督检验院 国家粮油及肉制品质量监督检验中心,郑州,450004 |
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| 基金项目： | 国家质检总局科技计划，河南省科技攻关项目 |
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| 摘 要： | 建立了基于负二阶导数红外光谱的反式脂肪酸的快速测定方法。以三反油酸甘油酯和三油酸甘油酯混合标样系列为校正集,使用带有水平衰减全反射(HATR)附件的傅里叶变换红外光谱仪采集校正集样品在1 050～900 cm-1波段范围的吸收光谱,求二阶导数,乘以-10 000,得负二阶导数谱(-2D-HATR-FTIR)。以970～960 cm-1波段范围的最大峰高为X轴,以三反油酸甘油酯含量为Y轴,构建定标方程为Y=0.348 8X-0.918 5,相关系数0.999 5。在0.5%～60%范围,验证集样品反式脂肪酸含量预测值与实际指定值显著相关,验证相关系数0.999 0,相对残差的绝对值小于10%,表明方法定标模型预测性能良好。方法检测下限达0.5%,改善了现有红外光谱法的灵敏度,适用于较低含量反式脂肪酸的快速测定。
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| 关 键 词： | 反式脂肪酸 傅里叶变换红外光谱 水平衰减全反射 负二阶导数 |
Rapid Determination of Trans Fatty Acids by Negative Second Derivative Horizontal Attenuated Total Reflection Fourier Transform Infrared Spectroscopy |
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| Tao Jian,Ding Taichun,Feng Bo,Jiang Yili,Yang Zhiling,Su Liang. Rapid Determination of Trans Fatty Acids by Negative Second Derivative Horizontal Attenuated Total Reflection Fourier Transform Infrared Spectroscopy[J]. Journal of the Chinese Cereals and Oils Association, 2011, 26(11): 115-118 |
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| Authors: | Tao Jian Ding Taichun Feng Bo Jiang Yili Yang Zhiling Su Liang |
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| Affiliation: | Tao Jian Ding Taichun Feng Bo Jiang Yili Yang Zhiling Su Liang (Henan Institute of Product Quality Inspection and Supervision,National Grain Oil & Meat-food Product Supervision and Inspection Center,Zhengzhou 450004) |
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| Abstract: | A rapid method was developed to determine trans fatty acids in foods using negative second derivative horizontal attenuated total reflection Fourier transform infrared spectroscopy(-2D-HATR-FTIR).Mixtures of trielaidin and triolaidin were prepared as a calibration set,the spectra in the region from 1 050 to 900 cm-1 was collected by the Fourier transform infrared spectroscopy with HATR accessory,to obtain a second derivative procedure,which was then multiplied with-10 000.Calibration plot and regression equ... |
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| Keywords: | trans fatty acids Fourier transform infrared spectroscopy horizontal attenuated total reflection negative second derivative |
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