| 真菌毒素人工抗原制备方法研究进展 |
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| 引用本文： | 吴春生,马良,胡媛媛,张宇昊.真菌毒素人工抗原制备方法研究进展[J].食品科学,2014,35(5):239-245. |
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| 作者姓名： | 吴春生 马良 胡媛媛 张宇昊 |
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| 作者单位： | 1.西南大学食品科学学院，重庆 400715；2.农业部农产品贮藏保鲜质量安全风险评估实验室（重庆），重庆 400716；3.重庆市特色食品工程技术研究中心，重庆 400716 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)项目(2013CB127803);国家自然科学基金项目(31301476);中央高校基本科研业务费专项资金项目(XDJK2013B035) |
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| 摘 要： | 基于免疫原理的酶联免疫吸附技术和金标免疫层析技术等是目前真菌毒素主要的快速检测技术。人工完全抗原的制备和抗体的性质是免疫快速检测技术中最关键的因素。本文对我国粮油产品危害较大的重点真菌毒素，综述目前国内外其各种人工抗原的制备/合成技术，并分析存在的问题和解决方法，为小分子污染物人工完全抗原的定向制备以及获得高亲和性高效价抗体提供一定的理论基础。
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| 关 键 词： | 真菌毒素 半抗原 人工抗原 制备 |
Recent Progress in the Preparation of Artificial Antigen of Mycotoxins |
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| WU Chun-sheng,MA Liang,HU Yuan-yuan,ZHANG Yu-hao.Recent Progress in the Preparation of Artificial Antigen of Mycotoxins[J].Food Science,2014,35(5):239-245. |
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| Authors: | WU Chun-sheng MA Liang HU Yuan-yuan ZHANG Yu-hao |
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| Affiliation: | 1. College of Food Science, Southwest University, Chongqing 400715, China; 2. Laboratory of Quality and Safety Risk Assessment
for Agro-products on Storage and Preservations (Chongqing), Ministry of Agriculture, Chongqing 400716, China;
3. Chongqing Special Food Programme and Technology Research Center, Chongqing 400716, China |
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| Abstract: | The main analytical techniques currently available for rapid detection of mycotoxins include enzyme-linkedimmunosorbent assay (ELISA) and gold-labeled immunochromatography. The synthesis of artificial antigen and the natureof antibodies are the most critical factors in rapid immunological detection of mycotoxins. This paper reviews the recentprogress at home and abroad in the preparation/synthesis of artificial antigens of mycotoxins as hazardous contaminantsof cereal and oil products. In addition, some existing problems and corresponding solutions are pointed out. This reviewwill hopefully provide a theoretical basis for preparing artificial antigens of small molecule contaminants and develop theantibodies with high affinity and specificity. |
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| Keywords: | mycotoxin hapten artificial antigen preparation |
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