EDC和NHS对玉米醇溶蛋白成膜性质的影响

为提高玉米醇溶蛋白膜的抗拉强度和伸长率，降低其水蒸气透过率和吸水率，研究交联剂1-乙基-3-（3-二甲基氨丙基）碳二亚胺盐酸盐（EDC）和N-羟基琥珀酰亚胺（NHS）对成膜性质的影响。结果表明：以90%乙醇为溶剂，EDC和NHS的添加量分别为0.06 g/g时，制得的膜性能最佳，抗拉强度为83 MPa，较未添加交联剂的蛋白膜提高97.6%；伸长率为5.5%，提高57.1%；水蒸气透过率为2.5×10-8（g·m）/（m2·h·Pa），降低43.2%；吸水率为39.4%，降低24.4%；质量损失率为3.6%，增加20.0%；静态接触角为67.4°，表明膜表面仍为疏水表面。原子力显微镜观测显示，加入交联剂后，蛋白分子聚集体变小，以均一的小球型聚集体形式紧密有序排列。

Effects of EDC and NHS on Physical Properties of Zein Films

The effects of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as crosslinking
reagents on the structure and properties of zein films were investigated to find ways to improve the tensile strength and
elongation and reduce the water vapor permeability and moisture absorption. Results showed that films prepared with 90% ethanol
as the solvent and 0.06 g of EDC/g zein or 0.06 g of NHS/g zein exhibited the best properties. The tensile strength, elongation,
water vapor permeability, moisture absorption and weight loss were 83 MPa, 5.5%, 2.5 × 10-8 (g·m)/ (m2·h·Pa), 39.4% and
3.6%, which were increased by 97.6%, 57.1%, reduced by 43.2% and 24.4%, and enhanced by 20.0% compared to those without
added cross-linking reagent, respectively, and the static water contact angle of the experimental samples was 67.4°, indicating the
film surface to be hydrophobic. In addition, the surface of zein films containing EDC and NHS was arranged orderly and closely as
smaller uniform spherical aggregates as observed using atomic force microscope (AFM)