固相萃取-高效液相色谱法同时测定海米中10 种合成色素

建立固相萃取-高效液相色谱法同时测定海米中新红、柠檬黄、日落黄、胭脂红、偶氮玉红、苋菜红、诱惑红、亮蓝、赤藓红、罗丹明B 10 种合成色素的方法。海米中的合成色素用氨化乙醇提取、固相萃取柱净化，ODS-3 C18柱为分离柱，甲醇和0.02 mol/L乙酸铵为流动相梯度洗脱，采用二极管阵列检测器，在360 nm波长处检测，外标法定量。10 种合成色素在0.2～50 mg/L质量浓度范围内与其峰面积成良好的线性关系；10 种合成色素的检出限为0.4～1.0 mg/kg。3 个不同加标水平的回收率为74.3%～90.0%，相对标准偏差小于4.3%。该方法能同时完成10 种合成色素的测定，可用于海米中合成色素的检测分析。

Simultaneous Determination of 10 Synthetic Pigments in Dried Shrimps Using Solid-Phase Extraction-High Performance Liquid Chromatography

A high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of 10
synthetic pigments (new red, lemon yellow, sunset yellow, carminum, azorubin, amaranth, allura red, light blue, erythrosine and
rhodamine B) in dried shrimps. Samples were extracted using aminated ethanol and cleaned up on a solid phase extraction column.
The chromatographic separation was achieved on an ODS-3 C18 column using a mobile phase consisting of methanol and 0.02 mol/L
ammonium acetate by gradient elution. Ten synthetic pigments were detected by a diode array detector at the wavelength of 360
nm and quantified by an external standard method. There was a good linear correlation between the concentrations of 10 synthetic
pigments and their peak areas in the range of 0.2–50 mg/L. The detection limits of 10 synthetic pigments were 0.4–1.0 mg/kg. The
recoveries were in the range from 74.3% to 90.0% and the RSDs were less than 4.3%.