葡萄籽原花青素对顺铂诱导人胚肾细胞凋亡的拮抗作用

目的： 探讨葡萄籽原花青素（ g r a p e s e e d p r o a n t h o c y a n i d i n ， G S P E ） 对顺铂（cis-diamminedichloroplatinum，CDDP）诱导人胚肾细胞HEK293凋亡及相关凋亡基因的影响，并探讨可能的机制。方法：体外培养正常HEK293细胞，噻唑蓝法测定GSPE、CDDP分别对HEK293细胞生长的影响以及GSPE对CDDP诱导HEK293细胞毒性的保护作用，流式细胞仪测定GSPE对CDDP所致HEK293细胞凋亡率的变化，Western blotting测定GSPE对CDDP所致HEK293细胞凋亡相关基因Bax、Bcl-2蛋白表达的影响。结果：GSPE对CDDP所致HEK293细胞毒性具有拮抗作用，可减轻CDDP所致HEK293细胞凋亡，减弱Bax表达、增强Bcl-2表达。结论：GSPE对CDDP所致HEK293细胞凋亡具有拮抗作用，其机制可能与GSPE降低促凋亡基因Bax，升高抗凋亡基因Bcl-2的表达有关。

Protective Effect of Grape Seed Proanthocyanidin Extract against Cisplatin-Induced Apoptosis in Human Embryonic Kidney Cells

Purpose: To explore the protective effect of grape seed proanthocyanidin extract (GSPE) against cisplatin
(CDDP)-induced apoptosis in human embryo kidney (HEK) 293 cells and its possible mechanisms. Methods: The effects of
CDDP and GSPE on the in vitro growth of HEK 293 cells and the effect of GSPE on CDDP-induced toxicity was evaluated
by MTT assay. CDDP-induced apoptosis in HEK293 cells was analyzed by flow cytometry. Western blotting was applied
to determine the expression of apoptotic gene Bax and anti-apoptosis gene Bcl-2. Results: GSPE was able to antagonize
cisplatin-induced cytotoxicity in a concentration-dependent manner. Flow cytometry revealed that GSPE could lead to a
decrease in apoptosis rate and pro-apoptotic Bax gene expression while anti-apoptotic gene Bcl-2 expression was enhanced.
Conclusion: GSPE demonstrates a preventive effect on cisplatin-induced injury in HEK293 cells. The mechanism may be
related to its capacity of enhancing anti-apoptotic gene Bcl-2 expression and inhibiting pro-apoptotic gene Bax expression.