大粒车前子多糖酸水解产物的分析

经水提、醇沉、Sevag除蛋白后得到精制大粒车前子多糖（Plantago asiatica L. polysaccharide，PLP），采用0.3 mol/L三氟乙酸对PLP进行部分酸水解。建立超高效液相色谱-蒸发光散射检测器技术筛选较优水解条件的方法，同时运用超高效液相色谱-四极杆飞行时间质谱联用法分析产物。结果表明：车前子多糖除蛋白后多糖含量为68.56%。在水解温度90 ℃、水解时间1.5 h条件下，能够得到比较多的水解产物，经超高效液相色谱-四极杆飞行时间质谱联用分析鉴定，水解产物中含有单糖、二糖至低聚六糖，并且存在戊糖和己糖相互连接的片段，但并未发现多个己糖相互连接的片段。

Product Analysis of Plantago asiatica L. Polysaccharide by Acid Hydrolysis

Polysaccharides were isolated from seeds of Plantago asiatica L. by water extraction and ethanol precipitation, and
subjected to protein removal by Sevag method, and then the polysaccharide sample was hydrolyzed with 0.3 mol/L TFA by partial
acid hydrolysis for different durations. An ultra performance liquid chromatography coupled with evaporative light scattering
detection (UPLC-ELSD) method was established for establishing better hydrolysis conditions, and ultra performance liquid
chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used for product analysis
of samples under the optimal hydrolysis conditions. Results showed that the content of polysaccharides in the deproteinized
sample was 68.56%. The optimal hydrolysis conditions were found to be hydrolysis with 0.3 mol/L TFA at 90 ℃ for 1.5 h. The
hydrolysate obtained under these conditions contained more degradation products like monosaccharides and oligosaccharides,
as identified by UPLC-Q-TOF-MS. Simultaneously, some pentose and hexose interconnected pieces in branch chains were also
detected. However, multiple hexose connected pieces were not observed.