响应面优化超声波提取猕猴桃根熊果酸工艺

为优化猕猴桃根熊果酸的超声波提取最佳工艺，在单因素试验基础上，选择乙醇体积分数、液料比、提取时间、提取温度为自变量，熊果酸得率为响应值，利用中心组合试验法和响应面分析法，研究各自变量交互作用及其对熊果酸得率的影响，模拟得到二次多项式回归方程的预测模型。结果表明：当超声功率225 W、超声频率40 kHz时，超声波提取熊果酸工艺最佳条件为乙醇体积分数73.8%、液料比7.80∶1（mL/g）、提取时间23.2 min、提取温度79.1 ℃。此提取条件下，熊果酸得率达到2.014%，与模型预测值之间具有较好的拟合性，且提取液中熊果酸的纯度为49.7%。

Optimization of Ultrasonic-Assisted Extraction of Ursolic Acid from Roots of Actinidia chinensis Using Response Surface Analysis

In this study, the conditions for ultrasonic-assisted extraction of ursolic acid (UA) from the roots of Actinidia
chinensis were optimized by response surface methodology. On the basis of single-factor experiments, a mathematical
regression model was established indicating the effect of ethanol concentration, solvent/solid ratio, extraction time and
temperature on the extraction yield of UA by response surface central composite design. Under the conditions of 225 W and
40 kHz, the optimum extraction conditions determined were as follows: 73.8% ethanol concentration, 7.80:1 solvent/solid
ratio (mL/g), 23.2 min and 79.1 ℃. Under these conditions, the experimental extraction yield of UA was 2.014%, which is
well matched with the predicted value, and the purity of UA in the extract was 49.7%.