苦荞异槲皮苷对人胃癌细胞SGC-7901增殖及凋亡的影响

从苦荞中提取制备异槲皮苷，研究其对人胃癌细胞SGC-7901增殖、凋亡、迁移和细胞周期的影响。将异槲皮苷作用于人胃癌SGC-7901细胞和人肾上皮细胞系293T，通过噻唑蓝法检测异槲皮苷对其增殖的影响；4’,6-二脒基-2-苯基吲哚(4’,6-diamino-2-phenyl indole，DAPI) 荧光染色法观察细胞核的形态学变化；划痕擦伤迁移实验检测异槲皮苷对SGC-7901细胞迁移能力的影响；流式细胞术检测异槲皮苷对SGC-7901细胞凋亡及细胞周期的影响。结果表明：苦荞异槲皮苷可以抑制SGC-7901细胞的增殖，并呈时间和剂量依赖性，当用100 μmol/L异槲皮苷作用细胞48 h后，对SGC-7901细胞的增殖抑制率达到35.92%， 而对人肾上皮细胞系293T的增殖抑制率仅为 3.15%；DAPI荧光染色法观察异槲皮苷处理细胞后，染色体凝聚，有凋亡小体产生；划痕擦伤实验显示，异槲皮苷能抑制SGC-7901细胞的迁移；流式细胞术检测结果表明，异槲皮苷可使G1和S期细胞减少，G2/M期细胞增多，且细胞凋亡率明显增加。综上所述，苦荞麦异槲皮苷能够诱导SGC-7901细胞发生凋亡，阻断细胞周期并抑制细胞增殖和迁移。

Effect of Isoquercetin from Fagopyrum tataricum on the Proliferation and Apoptosis of Human Gastric Carcinoma Cell Line SGC-7901

Isoquercetin was extracted from Fagopyrum tataricum and investigated for its effect on cell proliferation,
apoptosis and migration of human gastric carcinoma cell line SGC-7901. Cell proliferation was examined by using MTT
assay. The morphological changes of SGC-7901 were observed by DAPI nuclear staining. The effect of Fagopyrum
tataricum isoquercetin on cell migration was investigated by scratch-recovering tests in cell culture plates, as well as its
effect on cell apoptosis by flow cytometry. As a result, MTT assay showed that the isoquercetin could inhibit the viability
of SGC-7901 cells in time- and dose-dependent manners. SGC-7901 cell growth was inhibited by 35.92% or more at
isoquercetin concentration of 100 μmol/L for 48 h, compared to only 3.15% for 293T cells under the same conditions
of concentration and culture duration. DAPI nuclear staining showed chromosomal condensation and the production of
apoptotic bodies. Scratch-recovering tests indicated that the isoquercetin could suppress cell migration. Flow cytometry
showed that the number of cells at the G1 phase and S phase decreased and increased at the G2/M phase and the apoptotic cell
population exhibited a significant increase. Taken together, isoquercetin from F. tataricum can induce apoptosis, arrest cell
cycle and inhibit cell proliferation and migration of SGC-7901 cells.