P450(BM3) on steroids: the Swiss Army knife P450 enzyme just gets better |
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| Authors: | Wong Luet-Lok |
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| Affiliation: | Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, Oxford OX1 3QR, UK. luet.wong@chem.ox.ac.uk |
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| Abstract: | The catalytic regio- and stereoselective oxidation of aliphatic C-H bonds by using atmospheric dioxygen as the oxidant is a highly desirable reaction. Reetz and co-workers have recently reported the development of P450(BM3) mutants that oxidise testosterone with greatly increased turnover activity, total stereoselectivity and >95?% regioselectivity. |
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| Keywords: | directed evolution heme enzymes oxidation P450 monooxygenases steroids |
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