Allergic Skin Reactivity and Chemical Properties of Allergens in Two Grades of Lactose |
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| Authors: | SHUICHI KAMINOGAWA YOSHIHIRO KUMAGAI KUNIO YAMAUCHI EISAKU IWASAKI TOKUKO MUKOYAMA MINORU BABA |
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| Affiliation: | Authors Kaminogawa, Kumagai, and Yamauchi are with the Dept. of Agricultural Chemistry, Faculty of Agriculture, The Univ. of Tokyo, Tokyo 713, Japan. Authors Iwasaki, Mukoyama and Baba are with the Dept of Pediatrics, Doai Memorial Hospital, Tokyo 130, Japan. |
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| Abstract: | Skin tests of the nondialyzable fraction of lactose were performed on patients with a clinical diagnosis of cow's milk allergy. The yields of brownish-colored residue, allergens (ALG), were 0.006% from reagent grade lactose (ALG 1) and 0.011% from lactose used in a commercial infant food formula (ALG 2). The ALG was separated into four fractions by chromatography on Sephadex G-75. The highest incidences of positive skin reaction among the four ALG 1 and ALG 2 fractions were 8/9 and 8/12 respectively. The ALG fractions were identified as a sugar protein complex and the protein moiety of ALG contained high amounts of glutamic acid (or glutamine), threonin, asparagic acid (or asparagine) and proline. |
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