嗜酸乳杆菌胞外多糖提取工艺优化

微生物的胞外多糖是微生物在生长代谢过程中分泌到细胞壁外的黏液多糖或荚膜多糖,是具有增稠和胶体性质并能溶解或分散在水中的长链、高分子聚合物。试验以嗜酸乳杆菌胞外多糖为研究对象,对提取时间、提取的乙醇体积分数和提取的料液比进行单因素实验,并利用sas软件对其提取工艺进行优化,得到在80%体积分数的乙醇下,以1∶1.8(体积比)的料液比抽提12 h,得到的多糖的产量为1.91 g/L。

The Optimization of Extro-polysaccharides of Lactobacillus acidophilus in Extraction

Lactobacillus acidophilus,which is recognized as a safe fermentation creature,is probiotics which can stay in human intestinal wall.Many strains have been the stars in the food industry for a long time.The extro-polysacchrides of bacteria are macromolecule polymers which are secreted during the incubation or in the cell wall to protect the bacteria during incubation.The polysacchrides are diluted in the water.Its perfect characteristics in rheology and physics has put it on the stage for research and production.The extro-polysacchrides of lactobacii are not only important for flavor and quality of dairy,but also a secure source of polysaccharides,because of its capability of stabilizing and emulsifying the humidity of diary product.In this article,extraction of the extro-polysacchrides of lactobacillus acidophilus is optimized with the SAS software.The yield of polysaccharides is 1.91 g/L by extraction 80% ethanol(1:1.8) for 12hours.