| 蓖麻毒蛋白A链基因克隆与重复表达载体构建 |
| |
| 引用本文： | 黄凤兰,;李国瑞,;萨日娜,;陈永胜,;阎秀峰.蓖麻毒蛋白A链基因克隆与重复表达载体构建[J].中国油料,2009(2):167-172. |
| |
| 作者姓名： | 黄凤兰 ;李国瑞 ;萨日娜 ;陈永胜 ;阎秀峰 |
| |
| 作者单位： | [1]东北林业大学生命科学学院,哈尔滨黑龙江150000; [2]内蒙古民族大学生命科学学院,内蒙古通辽028000; [3]内蒙古民族大学农学院,内蒙古通辽028000 |
| |
| 基金项目： | 国家自然科学基因项目（30760123） |
| |
| 摘 要： | 利用降落PCR的方法，从蓖麻基因组DNA中克隆毒蛋白A链基因的560bp片段；利用一步法将该基因的2个正义片段和2个反义片段分别与pBI-121质粒连接，构建含该基因的正义重复和反义重复表达载体，为利用共抑制技术抑制蓖麻毒蛋白A链基因表达的研究奠定基础。
|
| 关 键 词： | 蓖麻毒蛋白 重复表达载体 降落PCR 一步法 共抑制 |
Ricin a chain gene cloning in Ricinus commol/Lunis L. and the construction of its repeat expression vectors |
| |
| Affiliation: | HUANG Feng - lan, LI Guo - rui, SA Ri - na, CHEN Yong - sheng, YAN Xiu - feng( 1. College of Life Science, Northeast Forestry University, Harbin, 150030, China ; 2. College of Life Science ,Inner Mongolia University for Nationalities, Tongliao ,028000, China ; 3. College of Agriculture, Inner Mongolia University for Nationalities, Tongliao , 028000, China) |
| |
| Abstract: | To clone the RTA gene from genome DNA of Ricinus commol/Lunis L. by touch down PCR. The expected 560bp gene fragment was obtained. Two RTA sense fragment and two RTA antisense fragment was linked to the plasmid pBI-121 respectively by one step. The sense and antisense repeat expression vector were constructed. This result provided a foundation for further research of RTA gene expression by co-suppression technology. |
| |
| Keywords: | Ricin Repeat expression vectors Touch down PCR One step Co-suppression |
| 本文献已被 维普 等数据库收录！ |
|
