| 高效液相色谱法-紫外检测器测定辣椒制品中辣椒素的含量 |
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| 引用本文： | 王燕,夏延斌,熊科,罗凤莲. 高效液相色谱法-紫外检测器测定辣椒制品中辣椒素的含量[J]. 食品科学, 2006, 27(9): 193-196 |
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| 作者姓名： | 王燕 夏延斌 熊科 罗凤莲 |
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| 作者单位： | 湖南农业大学食品科技学院 |
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| 基金项目： | 引进国际先进农业科技计划(948计划) |
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| 摘 要： | 本文研究了用超声波法提取辣椒素,再采用高效液相色谱法测定。色谱条件为:色谱柱为AgilentZorbaxSB-C18(5μm,250mm×4.6mm),柱温30.0℃。UV检测器的波长:280nm。流动相V(甲醇):V(水)=80:20,流速0.8ml/min,进样量10μl。结果表明用超声波提取法简单、提取率高。
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| 关 键 词： | 辣椒素 测定 高效液相色谱-紫外检测器(HPLC-UVD) |
| 文章编号： | 1002-6630（2006）09-0193-04 |
| 收稿时间： | 2005-10-18 |
| 修稿时间： | 2005-10-18 |
Determination of Capsaicinoid Content in Capsicum Products with HPLC-UVD |
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| WANG Yan,XIA Yan-bin,XIONG Ke,LUO Feng-lian. Determination of Capsaicinoid Content in Capsicum Products with HPLC-UVD[J]. Food Science, 2006, 27(9): 193-196 |
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| Authors: | WANG Yan XIA Yan-bin XIONG Ke LUO Feng-lian |
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| Affiliation: | College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China |
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| Abstract: | This article studied the extraction of capsaicin from capsicum products by ultrasonic processing, then the capsaicin was determined by high performance liquid chromatograph(HPLC).The HPLC system consisted of Agilent Zorbax SB-C18 (5μm,250mm×4.6mm), with column temperature 30℃, and the ultraviolet detector 280nm. The mobile phase was V(Methanol): V(H2O)=80:20 with a flow rate of 0.8ml/min, and the sample volume was 10μl. The result showed that the method of ultrasonic technique is easy, the extraction rate is high, and the result is satisfactory. |
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| Keywords: | capsaicin determination HPLC-UVD |
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