以中空纤维膜为成型模板制备壳聚糖微萃取探针

引言 固相微萃取技术(solid phase microextraction,SPME)[1]是一种新型的样品前处理技术,该技术是将高分子材料均匀地涂渍在石英纤维表面制成微萃取探针,在萃取时既继承了固相萃取技术的优点,又有效克服了容易堵塞的缺陷,且易于与GC[2]或HPLC[3]联用,具有快速、简便、浓缩倍数高、不消耗有机溶剂等优点,在样品前处理领域得到了广泛应用.

Preparation of chitosan microextraction probe with hollow fiber as shape template

Preparation of a novel coating is one of the keys to solid phase microextraction.A new method was proposed to prepare a chitosan microprobe with a film thickness of several dozen micrometers with the phase inverse technique by using a microporous polyacrylonitrile (PAN) hollow fiber with a diameter of 1.5 mm as template.The dilute acetic acid solution of chitosan and glutaraldehyde was added into the hollow fiber, a quartz fiber was inserted into the hollow fiber as supporter and then the hollow fiber was placed in the NaOH solution, because of the porosity of hollow fiber, NaOH diffused and contacted with acetic acid, then the chitosan thin film was formed.For intensifying the adsorption of acidic compounds, the probe was then modified with polyethyleneiminate (PEI).The SEM photos showed that the probe obtained with this process was 17 μm thick.The PEI-modified chitosan probes showed stronger adsorption of furomeside.Using NaOH as desorber, a concentration factor over 10 was observed and the solute concentration in the desorber was linear with that in the original samples.The operation is convenient and easy to realize automation.