Direct ELlSAs for Sulfathiazole in Milk and Honey with Special Emphasis on Enzyme Conjugate Preparation |
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| Authors: | CARRIE A THOMSON PETER SPORNS |
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| Affiliation: | Authors Thomson and Sporns are with the Dept. of Agricultural, Food &Nutritional Science, Univ. of Alberta, Edmonton, Alberta, Canada T6G 2P5. Address inquiries to Dr. Peter Sporns. |
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| Abstract: | Direct competitive enzyme-linked immunosorbent assays (ELISAs) were developed for sulfathiazole (ST) analysis. Polyclonal antibody rabbit serum adsorption to polystyrene microtiter plates was enhanced using a 1 μg/mL Protein A precoating solution. Detection limits (20% reduction in maximum enzyme signal) for ST in milk and honey were 12.0 and 34.7 ppb (ng/L), respectively. ST-horseradish peroxidase (HRP) conjugates prepared by linking the N4-amino group of ST to periodate-treated HRP were more efficient than those prepared using a more conventional two-step glutaraldehyde method. This represents a new method of preparing sulfonamide conjugates for use in ELlSAs. |
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| Keywords: | polyclonal antibody horseradish peroxidase milk honey sulfathiazole |
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