关于HCV不同区段抗原的反应性研究

目的　探讨各种丙型肝炎病毒 (HCV)抗原在诊断中的作用及其与HCVRNA的关系。方法　利用HCV不同区段抗原抗 -HCV酶联免疫试验 (EIA)和逆转录聚合酶链反应法 (RT PCR) ,对不同临床型HCV患者进行检测。结果　各组患者均以抗 NS3和抗 C阳性率最高。但均有一定比例患者抗 NS4和抗 NS5阳性。与各单个片段抗 HCV阳性率相比 ,抗 HCV总抗体阳性率最高。结论　NS3和C抗原在检测抗 HCV中起很重要作用 ,但NS4和NS5抗原在抗 HCVEIA中的作用也不容忽视。联合应用不同区段HCV抗原将大大提高抗 HCVEIA试剂的灵敏度。

Reactivity of Antigens Encoded by Different Gene Fragments of HCV

Objective To explore the roles of different HCV antigens in detection and the relationship between them with HCV RNA.Methods The serum samples from HCV carrier and the patients with acute,chronic hepatitis and HCV related hepatocellular carinoma were detected for the antibodies to antigens encoded by the different gene fragments of HCV and HCV RNA by EIA and RT PCR respectively.Results Compared with those of anti E1,E2,NS4 and NS5,the positive rates of anti NS3 and anti C were the highest in the 4 groups of patients.A proportion of the patients in each group was anti NS4 and/or anti NS5 positive.The positive rate of total anti HCV was much higher than that of antibody to the antigen encoded by any gene fragment of HCV.Conclusion NS3 and C antigens play important roles in the detection of anti HCV.However,the role of NS4 and NS5 antigens in EIA should not be omitted.Combined use of different HCV will improve the sensitivity of EIA significantly.