| 纳米羟基磷灰石颗粒固定化漆酶的研究木 |
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| 引用本文： | 黄宇,樊艳茹,张新慧,王显祥.纳米羟基磷灰石颗粒固定化漆酶的研究木[J].广州化工,2011,39(6):44-46,68. |
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| 作者姓名： | 黄宇 樊艳茹 张新慧 王显祥 |
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| 作者单位： | 1. 宁夏医科大学药学院,宁夏,银川,750004;宁夏回药现代化工程技术研究中心,宁夏,银川,750004
2. 四川农业大学生命科学与理学院,四川,雅安,625014 |
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| 基金项目： | 宁夏自然科学基金(NZ10106).宁夏医科大学面上项目 |
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| 摘 要： | 利用羟基磷灰石对漆酶进行了固定化研究,确定了羟基磷灰石对漆酶固定的最适条件,并研究了不同温度下游离漆酶与固定化漆酶活力的差异,同时也对游离漆酶与固定化漆酶的Km值进行了研究.结果表明:0.08 g羟基磷灰石固定1 mL粗酶液,当固定化过程pH为6,交联剂戊二醛浓度为6%,固定化时间为1 h时,酶活力达到最大.当温度在3...
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| 关 键 词： | 羟基磷灰石 固定化酶 漆酶 |
Research of Immobilized Laccase on Nano-Hydroxyapatite Particles |
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| HUANG Yu,FAN Yan-ru,ZHANG Xin-hui,WANG Xian-xiang.Research of Immobilized Laccase on Nano-Hydroxyapatite Particles[J].GuangZhou Chemical Industry and Technology,2011,39(6):44-46,68. |
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| Authors: | HUANG Yu FAN Yan-ru ZHANG Xin-hui WANG Xian-xiang |
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| Affiliation: | 1 Pharmacy College,Ningxia Medical University,Ningxia Yinchuan 750004;2 Ningxia Research Center of Modern Hui Medicine Engineering and Technology,Ningxia Yinchuan 750004;3 College of Life and Science,Sichuan Agricultural University,Sichuan Ya’an 625014,China) |
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| Abstract: | Immobilization of laccase to hydroxyapatite was researched,and the best condition of immobilization was confirmed.Otherwise,the distinction on the activity between immobilized laccase and dissociative laccase and the two laccases’Km were studied.The results showed that 1 mL original enzyme solution immobilization on 0.08 g hydroxyapatite,if the pH was 6.0,the concentration of glutaraldehyde was 6%,and the time of the immobilization was 1 h,the activity of the immobilized laccase would be the highest.The activity of the dissociative laccase would be increasing if the temperature ranges was 30~70 ℃.The activity of the immobilized laccase would be the highest when the temperature was 50 ℃. |
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| Keywords: | hydroxyapatite immobilized enzyme laccase |
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