Positional distribution of FA in TAG of enzymatically modified borage and evening primrose oils |
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Authors: | Senanayake S P J Namal Shahidi Fereidoon |
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Affiliation: | (1) Department of Biochemistry, Memorial University of Newfoundland, A1B 3X9 St. John's, Newfoundland, Canada |
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Abstract: | Stereospecific analysis was carried out to establish positional distribution of FA in the TAG of DHA, EPA, and (EPA+DHA)-enriched
oils. In this study, TAG of enzymatically modified oils were purified using a silicic acid column. The TAG were then subjected
to positional distribution analysis using a modified procedure involving reductive cleavage with Grignard reagent. The results
showed that in DHA-enriched borage oil (BO), DHA was randomly distributed over the three positions of TAG, whereas γ-linolenic
acid (GLA) was mainly esterified at the sn-2 and-3 positions. In DHA-enriched evening primrose oil (EPO), however, DHA and GLA were concentrated in the sn-2 position. In EPA-enriched BO, EPA was randomly distributed over the three positions of TAG, similar to that observed for
DHA. In EPA-enriched EPO, however, this FA was mainly located at the primary positions (sn-1 and sn-3) of TAG. In both oils, GLA was preferentially esterified at the sn-2 position. In (EPA+DHA)-enriched BO, EPA and DHA were mainly esterified at the sn-1 and -3 positions of TAG, whereas GLA was mainly located at the sn-2 position. In (EPA+DHA)-enriched EPO, GLA was mainly located at the sn-2 and-3 positions; EPA was preferentially esterified at the sn-1 and-3 positions, and DHA was found mainly at the sn-3 position. |
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