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溶菌酶与矿物粉尘反应行为的模拟研究
引用本文:白进伟,迟燕华,胡亚敏. 溶菌酶与矿物粉尘反应行为的模拟研究[J]. 四川建材学院学报, 2009, 0(2): 70-73
作者姓名:白进伟  迟燕华  胡亚敏
作者单位:西南科技大学材料科学与工程学院,四川绵阳621010
基金项目:国家自然科学基金(400720201)、教育部科学技术研究重点项目(02126)资助.
摘    要:对3个地区的矿物粉尘做了XRF分析及其与溶菌酶(Lys)的反应实验,结果显示Lys与样品中的物质进行了反应。在模拟样品中元素离子与Lys反应的uV—Vis、荧光光谱研究中发现,37℃下Lys与样品中的元素离子Fe^3+、Pb^2+的作用很明显。表现在紫外区Lys—Fe^3+复合物吸光度增强,Lys—Pb^2+复合物吸收峰红移13nm。荧光区Lys—Fe^3+复合物在λem450nm处荧光强度增强,荧光峰红移30nm,Lys—Pb^2+复合物在λem725nm处荧光强度增强,荧光峰红移205nm。

关 键 词:溶菌酶  矿物粉尘  UV—Vis光谱  荧光光谱

Simulated Study on the Reaction between Lysozyme and Fiber Dusts
BAI Jin-wei,CHI Yan-hua,HU Ya-min. Simulated Study on the Reaction between Lysozyme and Fiber Dusts[J]. , 2009, 0(2): 70-73
Authors:BAI Jin-wei  CHI Yan-hua  HU Ya-min
Affiliation:(School of Materials Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Siehuan, China)
Abstract:Fiber dusts of three different areas were analyzed by XRF and samples were reacted with Lysozyme. The results show that Lysozyme has reacted with the substances in the samples. The UV-Vis and Fluorescent Spectra study was conducted on the reaction between the simulated ion and Lys, which found that the reaction between Lysozyme and element Fe^3+ , Pb^2+ in the samples was obvious at 37℃. In UV- vis spectra the Lys-Fe^3+ absorption was strengthened and the Lys-Pb^2+ maximum absorption peak red shifted 13 nm. In Fluorescent spectra, the Lys-Fe3+maximum fluorescence intensity appeared at the λem 450 nm and the fluorescence peak red shifted 30 nm, the Lys-Pb^2+ maximum fluorescence intensity appeared at the λem 725 nm and the fluorescence peak red shifted 205nm.
Keywords:Lysozyme  Fiber dusts  UV-Vis spectrum  Fluorescence spectrum
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