| ATF1过表达和BAT2敲除酿酒酵母发酵性能的研究 |
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| 引用本文： | 刘玉兰,张翠英,张燕,汪东升,肖冬光. ATF1过表达和BAT2敲除酿酒酵母发酵性能的研究[J]. 中国酿造, 2012, 31(1): 151-153 |
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| 作者姓名： | 刘玉兰 张翠英 张燕 汪东升 肖冬光 |
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| 作者单位： | 工业发酵微生物教育部重点实验室,天津市工业微生物重点实验室,天津科技大学生物工程学院,天津300457 |
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| 基金项目： | 天津市高等学校科技发展基金计划项目 |
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| 摘 要： | 以啤酒酵母工业菌株S5为对照,对过表达醇乙酰基转移酶编码基因ATF1同时敲除氨基酸转氨酶编码基因BAT2酿酒酵母工程菌株S5-1进行发酵性能的研究。结果表明,与出发菌株相比,突变株生长状况、发酵速度、酒精度等基本发酵性能没有明显变化,而突变株发酵后的乙酸酯总量有较大程度的提升,为85.44mg/L,提高了6.96倍,高级醇总量有较大程度的下降,为79.25mg/L,降低了27.28%。研究结果为啤酒风味的改善奠定了良好的基础。
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| 关 键 词： | 啤酒酵母 发酵性能 乙酸酯 高级醇 |
Fermentation performances of Saccharomyces cerevisiae with A TF1 overexpression and BAT2 knock-out |
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| LIU Yulan , ZHANG Cuiying , ZHANG Yan , WANG Dongsheng , XIAO Dongguang. Fermentation performances of Saccharomyces cerevisiae with A TF1 overexpression and BAT2 knock-out[J]. China Brewing, 2012, 31(1): 151-153 |
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| Authors: | LIU Yulan ZHANG Cuiying ZHANG Yan WANG Dongsheng XIAO Dongguang |
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| Affiliation: | (Key Laboratory of Industrial Microbiology,Ministry of Education,Key Laboratory of Tianjin Industrial Microbiology,College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China) |
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| Abstract: | Fermentation performances of the parental strain S5 and the mutant that with alcohol overexpressed acetyltransferase gene encoding ATF1 and knocked out amino acid transaminase gene encoding BAT2 were tested.The results showed that,compared to the parental strain,the total amount of acetic esters produced by the mutant was 85.44mg/L,6.96 times as that of the parental strain,the yield of higher alcohols of the mutant decreased to 79.25mg/L,reduced by 27.28%,while alcohol content,fermentation rate and other fermentation characters did not change significantly.The results gave a good foundation for the improvement of beer aroma. |
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| Keywords: | beer yeast fermentation performances acetic esters higher alcohols |
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