利用响应面法优化溶杆菌UCo1产溶菌酶的发酵培养基

采用响应面法对溶杆菌UCo1产溶菌酶的培养基进行了优化。首先对溶杆菌UCo1产溶菌酶的发酵培养基进行了单因素试验,确定了影响产酶的3个显著因素,即碳源麦芽糖,氮源大豆蛋白胨和表面活性剂Tween-20。采用Box-Behnken响应面法对溶菌酶的发酵培养基组成进行了优化,确定了最佳条件。结果表明,麦芽糖,大豆蛋白胨和Tween-20 3因素的最佳浓度分别为1.725%,3.25%,0.048%时,溶菌酶酶活达到6973.5U/mL,与模型所得到的最大预测值6990.99U/mL基本吻合,且较优化前的酶活力(6230.4U/mL)提高了11.93%。

Optimization of fermentation medium producing lysozyme by Lysobacter UCo1 using response surface methodology

Response surface analysis was applied to optimize the fermentation medium for lysozyme production by Lysobacter UCO1.In the first step,single factor tests were used to evaluate the effect of four related factors on fermentation medium producing lysozyme,and three main factors were screened out.Box-Behnken design and response surface analysis were used to further investigate the optimal central levels and relationships of these factors.Finally,the optimal combined fermentation conditions for maximum enzyme activity were determined as follows： malt sugar 1.725%,soybean peptone 3.25%,and Tween-20 0.048%.Under the optimization of culture conditions,the maximum lysozyme activity was 6973.5U/ml which was in good agreement with the predicted value（6990.99U/ml） and was increased by 11.93% compare to that before optimization.