| 长双歧杆菌活菌制剂下游制备方法优化 |
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| 引用本文： | 邵建宁,张文齐,麻和平,刘彩云,慕婷婷,魏亚琴,赵昊星.长双歧杆菌活菌制剂下游制备方法优化[J].中国酿造,2012,31(1):99-101. |
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| 作者姓名： | 邵建宁 张文齐 麻和平 刘彩云 慕婷婷 魏亚琴 赵昊星 |
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| 作者单位： | 甘肃省科学院生物研究所,甘肃兰州730000 |
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| 基金项目： | 甘肃省科学院应用技术研究与开发计划项目 |
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| 摘 要： | 对长双歧杆菌活菌制剂制备中的菌体收集、保护剂和冷冻干燥工艺进行研究,优化了菌体收集及冷冻干燥工艺条件,比选出有效的长双歧杆菌保护剂。采用低温离心收集菌体、添加保护剂及真空冷冻干燥获得了适合的长双歧杆菌活菌制剂下游制备方法。
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| 关 键 词： | 长双歧杆菌 菌体收集 冷冻干燥 保护剂 |
Optimization of downstream process for viable-preparation of Bifidobacterium longum |
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| SHAO Jianning
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ZHANG Wenqi
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MA Heping
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LIU Caiyun
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MU Tingting
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WEI Yaqin
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ZHAO Haoxing.Optimization of downstream process for viable-preparation of Bifidobacterium longum[J].China Brewing,2012,31(1):99-101. |
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| Authors: | SHAO Jianning ZHANG Wenqi MA Heping LIU Caiyun MU Tingting WEI Yaqin ZHAO Haoxing |
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| Affiliation: | (Institute of Biology,Gansu Academy of Sciences,Lanzhou 730000,China) |
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| Abstract: | Cell collection,cryoprotectant and freeze-drying process for viable-preparation of Bifidobacterium longum were optimization in this paper.Effective cryoprotectant of B.longum was selected.Optimal downstream process was obtained through low temperature centrifugation,adding cryoprotectant and vacuum freeze-drying. |
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| Keywords: | Bifidobacterium longum cell collection freeze-drying cryoprotectant |
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