| 堆积糖化对玉米秸秆同步糖化固态发酵生产乙醇的影响 |
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| 引用本文： | 牛堃,赵华,夏媛媛,董晓宇. 堆积糖化对玉米秸秆同步糖化固态发酵生产乙醇的影响[J]. 中国酿造, 2012, 31(4): 102-105 |
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| 作者姓名： | 牛堃 赵华 夏媛媛 董晓宇 |
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| 作者单位： | 工业微生物教育部重点实验室,天津科技大学生物工程学院,天津300457 |
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| 摘 要： | 该文以玉米秸秆为原料,经蒸汽爆破预处理后接入Trichoderma reesei Rut C-40培养纤维素酶曲,将纤维素酶曲与汽爆秸秆混合堆积糖化后,接入酵母菌进行同步糖化固态发酵生产乙醇,通过Box-Behnken设计实验得到最适酶解工艺条件:酶曲/汽爆秸秆为1.2,温度46℃,pH值4.4,堆积糖化48h后酶解率可达到32.50%。将酶解糖化48h后的底物接入酵母菌,发酵96h后乙醇产率可达0.15g/g底物,较直接同步糖化发酵乙醇产率提高了9.3%。
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| 关 键 词： | 玉米秸秆 堆积 同步糖化发酵 乙醇 纤维素酶 响应面 |
Effect of simultaneous saccharification and fermentation on ethanol production from corn stalk after accumulation |
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| NIU Kun , ZHAO Hua , XIA Yuanyuan , DONG Xiaoyu. Effect of simultaneous saccharification and fermentation on ethanol production from corn stalk after accumulation[J]. China Brewing, 2012, 31(4): 102-105 |
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| Authors: | NIU Kun ZHAO Hua XIA Yuanyuan DONG Xiaoyu |
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| Affiliation: | (Key Laboratory of Industrial Fermentation Microbiology,College of Biotechnology,Tianjin University of Science and Technology, Tianjin 300457,China) |
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| Abstract: | In this study,Trichoderma reesei Rut C-40 was inoculated to the steam-explosion corn stalks to produced cellulase.Then the yeasts were cultivated in the mixture of cellulase and steam-explosion stalk to produce ethanol.The optimal conditions for enzymatic hydrolysis were determined by Box-Behnken,which were the proportion of cellulase to steam-explosion stalk was 1.2,temperature 46℃ and pH value 4.4.hydrolysis yield was 32.50% after 48h saccharification.When the yeasts were inoculated to the substrates,the productivity of ethanol was 0.15g/g substrate after 96h,which was 9.3% higher than the yield of SSF without saccharification. |
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| Keywords: | corn stalk accumulation simultaneous saccharification and fermentation ethanol cellulase response surface |
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