| 荷叶离褶伞中试发酵条件与培养基优化研究 |
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| 引用本文： | 张汉燚,王治江,席亚丽,王小明,魏生龙. 荷叶离褶伞中试发酵条件与培养基优化研究[J]. 中国酿造, 2012, 31(1): 96-99 |
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| 作者姓名： | 张汉燚 王治江 席亚丽 王小明 魏生龙 |
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| 作者单位： | 河西学院食用菌研究所,甘肃张掖,734000 |
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| 基金项目： | 科技部农业科技成果转化基金项目，甘肃省科技支撑项目 |
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| 摘 要： | 在荷叶离褶伞菌丝体摇瓶发酵条件研究结果的基础上,采用均匀设计,在200L发酵罐中,对荷叶离褶伞中试发酵条件与培养基配方进行了优化研究,结果表明荷叶离褶伞菌丝体中试发酵的最佳条件为搅拌转速160r/min、罐压0.2MPa、通气量80L/h、pH值为6.5、温度20℃、接种量10%;最佳培养基配方为玉米面5%、大豆0.5%、ZnSO4 0.025%、MgSO4 0.05%、KH2PO4 0.05%。发酵至第8d菌丝体生物量达到最大(10.578g/L),第9d胞外多糖(exopolysaccharides,EPS)产量最大(1.212g/L)。
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| 关 键 词： | 荷叶离褶伞 菌丝体生物量 胞外多糖 中试 |
Optimization of fermentation conditions and medium components for pilot production of Lyophyllum decastes |
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| ZHANG Hanyi , WANG Zhijiang , XI Yali , WANG Xiaoming , WEI Shenglong. Optimization of fermentation conditions and medium components for pilot production of Lyophyllum decastes[J]. China Brewing, 2012, 31(1): 96-99 |
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| Authors: | ZHANG Hanyi WANG Zhijiang XI Yali WANG Xiaoming WEI Shenglong |
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| Affiliation: | (Mushroom Research Institute,Hexi University,Zhangye 734000,China) |
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| Abstract: | Based on the previous results of shake-flask fermentation conditions of Lyophyllum decastes mycelia,fermentation conditions and medium components of L.decastes Mycelia were optimized by uniform design with 200L fermenter.The results showed that optimum conditions of pilot fermentation were as follows： agitation speed 160r/min;fermenter pressure 0.2MPa;ventilation 80L/h;pH value 6.5;temperature 20℃;and inoculum size 10%.The optimum medium components were as follows： corn flour 5%;soybean 0.5%,ZnSO4 0.025%;MgSO4 0.05%;and KH2PO4 0.05%.Yield of mycelia was maximum on the eighth day（10.578g/L）,and the content of exopolysaccharides was maximum on the ninth day（1.212g/L）. |
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| Keywords: | Lyophyllum decastes mycelia yield exopolysaccharides pilot |
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