| 固定枯草芽孢杆菌发酵生产类细菌素的研究 |
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| 引用本文： | 马莉,罗俊成,张宿义,王忠彦,胡承. 固定枯草芽孢杆菌发酵生产类细菌素的研究[J]. 食品与发酵工业, 2006, 32(11): 39-42 |
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| 作者姓名： | 马莉 罗俊成 张宿义 王忠彦 胡承 |
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| 作者单位： | 1. 四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都,610064
2. 泸州老窖股份有限公司,泸州,646000 |
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| 摘 要： | 以海藻酸钙为材料,固定枯草芽孢杆菌(Bacillussubtilis)LL18-4,研究了不同条件下类细菌素效价的变化情况。结果表明,利用3%海藻酸钠在2%CaCl2条件下,得到固定化细胞颗粒的类细菌素效价和稳定性较好,可维持216h无破裂;对其固定化颗粒进行发酵条件的研究发现,培养温度37℃,pH值6.5,接种量1%,72h静止培养,固定菌所产类细菌素的抑菌活性高,最高效价为334.2u/mL。通过216h3批次循环的半连续培养后,固定菌活性仍能维持在260.9u/mL以上。
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| 关 键 词： | 枯草芽孢杆菌 类细菌素 海藻酸钙 固定化细胞 |
| 收稿时间： | 2006-08-09 |
| 修稿时间： | 2006-09-15 |
Study on Bacteriocin-like Substance Fermentation by Immobilized Bacillus subtilis Cells |
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| Ma Li,Luo Juncheng,Zhang Suyi,Wang Zhongyan,Hu Cheng. Study on Bacteriocin-like Substance Fermentation by Immobilized Bacillus subtilis Cells[J]. Food and Fermentation Industries, 2006, 32(11): 39-42 |
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| Authors: | Ma Li Luo Juncheng Zhang Suyi Wang Zhongyan Hu Cheng |
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| Affiliation: | 1.School of Life Science, Sichuan University, Key Laboratory of Bio-resources and Eco-environment Ministry of Education, Chengdu 610064, China; 2.Luzhou Laojiao Co Ltd. , Luzhou 646000, China |
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| Abstract: | Attempts were made to produce bacteriocin-like substance by immobilized cells of Bacillus subtilis LL18-4 entrapped in calcium alginate beads.The results showed that the immobilized cell beads were intact for 216 h under 3% of calcium alginate in 2% CaCl2 solution.The physical stability of beads was improved and the following fermentation conditions were the best for Immobilized cells to produce bacteriocin-like substance of the highest activity:culture temperature 37℃,pH 6.5,1% inoculation quantity,incubated 72 h culture time and valence as 334.2 u/mL.The bacteriocin-like substance activity was more than 260.9 u/mL after the third cycles. |
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| Keywords: | Bacillus subtilis bacteriocin-like substance calcium alginate immobilized cells |
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