槲皮素-锌(Ⅱ)配合物体内外抑制亚硝胺合成的研究

为改善槲皮素的水溶性,将其制成锌(II)配合物,在模拟人体胃液(pH3.0,37℃)的条件下,通过测定N-二甲基亚硝胺(NDMA)的阻断率、NO2-的形成量和清除率研究了槲皮素-锌(II)配合物体外对亚硝化反应的抑制活性;以血清谷丙转氨酶为测定指标,通过动物实验考察了槲皮素-锌(II)配合物体内对亚硝胺合成的阻断作用。结果表明:槲皮素-锌(II)配合物能明显阻断亚硝胺的化学合成,其最大阻断率为91.70%,对亚硝酸钠具有较好的清除活性,最大清除率为80.64%;其对硝酸盐还原酶还原NO3-为NO2-的抑制率可达58.64%。体内阻断亚硝胺合成的实验中,槲皮素-锌(II)配合物组血清谷丙转氨酶显著低于对照组(p<0.01)。

Study on Inhibition Effects of Quercetin-zinc Complex on Nitrosamine Synthesis in vivo and in vitro

To improve the solubility of quercetin in water,quercetin-zinc complex was synthesized.The activity of quercetin-zinc complex on inhibiting nitrosation was studied by blocking rate on synthesis of nitrosamine,formation of nitrite ion and scavenging rate under the condition of simulated gastric juice(pH3.0,37 ℃)in vitro in this paper.The activity of blocking synthesis of nitrosamine by experimental of small mice was investigsted in vivo,taking serum GPT as index.The results showed that quercetin-zinc complex has strong capability of blocking nitrosamine synthesis.The maximum blocking rate of NDMA is 91.70%,and the activity of scavenging sodium nitrite is obvious.The maximum eliminating rate of nitrite sodium is 80.64%.Quercetin-zinc complex inhibited nitrate ion reduced into nitrite ion by nitrate reductase,and the inhibitory rate amounted to 58.64%.During the process of blocking synthesis of nitrosamine,the serum GPT in quercetin-zinc complex group is significantly lower than those in control group(p<0.01).