UPLC-MS-MS法测定八宝粥罐头中乙二胺四乙酸二钠残留量

建立超高效液相色谱-串联质谱法测定八宝粥罐头中乙二胺四乙酸二钠（ethylenediaminetetraacetic aciddisodium，EDTA-2Na）残留量的检测和确证方法。试样中残留的EDTA-2Na用水高速匀浆提取，提取液经三氯化铁衍生化、三氯甲烷和MAX阴离子交换柱净化后，用超高效液相色谱-串联质谱仪检测和确证，外标法定量。EDTA-2Na质量浓度在1.0～50.0 μg/mL范围内时，线性关系良好，EDTA-2Na的相关系数（R2）为0.992 2。在20～400 mg/kg添加范围内，样品平均加标回收率在79.4%～105.3%之间，相对标准偏差为7.93%～9.94%。EDTA-2Na的最低检出限为20 mg/kg。该方法具有灵敏、准确、稳定等优点，可用于八宝粥罐头中EDTA-2Na残留量测定。

Determination of Ethylenediaminetetraacetic Acid Disodium Residues in Canned Eight-Ingredient Porridge by UPLC-MS-MS

An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) method was developed
for the determination and confirmation of ethylenediaminetetraacetic acid disodium (EDTA-2Na) residues in canned
eight-ingredient porridge. The EDTA-2Na residues in the test samples were extracted with water by means of high-speed
homogenization. The extract was derivatized with FeCl3 reagent, cleaned up by chloroform and MAX anion exchange
column. The residues were determined and confirmed by UPLC-MS-MS using an external standard method. The linear range
was 1.0–50.0 μg/mL for EDTA-2Na with a correlation coefficient (R2) of 0.992 2. The average recoveries of EDTA-2Na in
spiked samples ranged from 79.4%–105.3%, with relative standard deviations between 7.93% and 9.94% at spiked levels
of 20–400 mg/kg. The limit of detection was 20 mg/kg for EDTA-2Na. The method is sensitive, accurate, repeatable and
suitable for the determination of EDTA-2Na residues in canned eight-ingredient porridge.