| 转基因大豆MON89788实时荧光PCR检测方法的建立 |
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| 引用本文： | 刘欣,张国丛,周兴虎,祝长青,黄明.转基因大豆MON89788实时荧光PCR检测方法的建立[J].食品科学,2015,36(4):193-197. |
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| 作者姓名： | 刘欣 张国丛 周兴虎 祝长青 黄明 |
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| 作者单位： | 1.南京农业大学食品科技学院，南京肉制品加工产业创新中心，江苏 南京 210095；
2.石家庄市农林科学研究院，河北 石家庄 050041；3.南京佳邦食品有限公司，江苏 南京 211225 |
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| 基金项目： | 公益性行业(农业)科研专项(201303083-2);江苏省科技支撑计划项目(BE2014304);南京市生物农业项目(2013) |
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| 摘 要： | 为实现转基因大豆MON89788的标识管理，针对转基因大豆MON89788的品系特异性序列设计引物和TaqMan探针，建立转基因大豆MON89788实时荧光聚合酶链式反应（polymerase chain reaction，PCR）检测方法，并对该方法的特异性、灵敏度和重复性进行检测。结果显示：建立的转基因大豆MON89788实时荧光PCR检测方法能扩增出127 bp的产物，特异性强，灵敏度达到0.1%，约为40 个单倍体基因组拷贝，检测重复性好，可成功应用于实际样品检测。因此，建立的转基因大豆MON89788实时荧光PCR检测方法可以应用于转基因大豆MON89788大豆及其制品的检测。
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| 关 键 词： | 转基因大豆 MON89788品系 实时荧光PCR 品系特异性检测 转基因标识 |
Establishment of Event-Specific Real-Time PCR Method for Detection of Genetically Modified Soybean MON89788 |
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| LIU Xin;ZHANG Guocong;ZHOU Xinghu;ZHU Changqing;HUANG Ming.Establishment of Event-Specific Real-Time PCR Method for Detection of Genetically Modified Soybean MON89788[J].Food Science,2015,36(4):193-197. |
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| Authors: | LIU Xin;ZHANG Guocong;ZHOU Xinghu;ZHU Changqing;HUANG Ming |
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| Affiliation: | 1. Nanjing Innovation Center of Meat Products Processing, College of Food Science and Technology,Nanjing Agricultural University, Nanjing 210095, China; 2. Shijiazhuang Academy of Agricultural and Forestry Sciences,Shijiazhuang 050041, China; 3. Nanjing Jiabang Food Co. Ltd., Nanjing 211225, China |
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| Abstract: | For implementation of labeling regulations, an event-specific real-time PCR method for the detection of
genetically modified soybean MON89788 was established in this study. Primers and TaqMan probe were designed based on
the event-specific sequence of MON89788 soybean. The specificity, sensitivity and repeatability of the developed method
were examined, respectively. The results showed that the method could amplify a 127 bp product specifically; the limit of
detection was 0.1% in 50 ng of soybean genomic DNA, approximately corresponding to 40 soybean haploid genome copies;
and this method had good repeatability and could be successfully applied to the detection of real samples. These results
indicated that the strain-specific real-time PCR method was suitable for the identification of genetically modified soybean
MON89788 and its products. |
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| Keywords: | genetically modified soybean MON89788 event real-time PCR event-specific detection transgenic labeling |
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