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Direct characterisation of phenolic antioxidants in infusions from four Mapuche medicinal plants by liquid chromatography with diode array detection (HPLC-DAD) and electrospray ionisation tandem mass spectrometry (HPLC-ESI-MS) |
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| Authors: | Mario J. Simirgiotis,Mario SilvaJosé Becerra,Guillermo Schmeda-Hirschmann |
| Affiliation: | a Departamento de Ciencias y Tecnología Vegetal, Universidad de Concepción, Campus Los Ángeles, J. A. Coloma 201, Los Ángeles, Chile b Laboratorio de Productos Naturales, Departamento de Química, Facultad de Ciencias Básicas, Universidad de Antofagasta, Camino a Coloso S/N, Antofagasta 02800, Chile c Departamento de Botánica. Facultad de Ciencias Naturales y Oceanográficas. Universidad de Concepción, Casilla 160, Concepción, Chile d Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Universidad de Talca, Casilla 747, Talca, Chile |
| Abstract: | A simple, fast and direct method was developed for the qualitative analysis of phenolic constituents from infusions of Mapuche medicinal plants. Teas made of Linum chamissonis Schiede, Quinchamalium chilensis Mol., Adesmia emarginata Clos. and Escallonia illinita K. Presl. were analysed by high-performance liquid chromatography with diode array detector (HPLC-DAD) and electrospray mass spectrometry (ESI-MS). This technique allowed for the first time the tentative identification of 16 phenolic compounds in E. illinita, 27 in Q. chilensis, 10 in L. chamissonis and 19 in A. emarginata. The compounds were mainly phenolic acids, flavonoid glycosides, anthocyanins and tannins. The total phenolic and flavonoid content of the infusions was assessed as well as the free radical scavenging capacity measured by the bleaching of a solution of the 2,2-diphenyl-1-picrylhydrazyl (DPPH−) radical. From the four species, Q. chilensis exhibited the strongest antioxidant activity with highest total phenolic and flavonoid content. |
| Keywords: | Adesmia emarginata Escallonia illinita Linum chamissonis Quinchamalium chilensis Mapuche medicinal plants Antioxidant Phenolics High performance liquid chromatography Electrospray ionisation-mass spectrometry |
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