Relevance of calpain and calpastatin activity for texture in super-chilled and ice-stored Atlantic salmon (Salmo salar L.) fillets |
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Authors: | MØ Gaarder D BahuaudE Veiseth-Kent T MørkøreMS Thomassen |
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Affiliation: | a Norwegian University of Life Sciences (UMB), Department of Animal- and Aquacultural Sciences (IHA), Post Box 5003, 1432 Aas, Norway b Nofima Mat AS, Osloveien 1, 1430 Aas, Norway c Nofima Marin AS, Osloveien 1, 1430 Aas, Norway |
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Abstract: | The aim of the present experiment was to measure the protease activities in ice-stored and super-chilled Atlantic salmon (Salmo salar) fillets, and the effect on texture. Pre-rigour fillets of Atlantic salmon were either super-chilled to a core temperature of −1.5 °C or directly chilled on ice prior to 144 h of ice storage. A significantly higher calpain activity was detected in the super-chilled fillets at 6 h post-treatment compared to the ice-stored fillets and followed by a significant decrease below its initial level, while the calpastatin activity was significantly lower for the super-chilled fillets at all time points. The cathepsin B + L and B activities increased significantly with time post-treatment; however, no significant differences were observed at any time points between the two treatments. For the ice stored fillets, the cathepsin L activity decreased significantly from 6 to 24 h post-treatment and thereafter increased significantly to 144 h post-treatment. There was also a significantly lower cathepsin L activity in the super-chilled fillets at 0 h post-treatment. No significant difference in breaking force was detected; however, a significant difference in maximum compression (Fmax) was detected at 24 h post-treatment with lower Fmax in the super-chilled fillets. This experiment showed that super-chilling had a significant effect on the protease activities and the ATP degradation in salmon fillets. The observed difference in Fmax may be a result of these observed differences, and may indicate a softening of the super-chilled salmon muscle at 24 h post-treatment. |
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Keywords: | Atlantic salmon ATP Calpains Calpastatin Cathepsins Texture Quality |
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