Germination induces the glucosylation of the Fusarium mycotoxin deoxynivalenol in various grains |
| |
Authors: | Ronald Maul Christian MüllerStephanie Rieß Matthias KochFrank-Jürgen Methner Nehls Irene |
| |
Affiliation: | a BAM Federal Institute for Materials Research and Testing, Division Organic Chemical Analysis, Reference Materials, Richard-Willstätter-Straße 11, 12489 Berlin, Germany b Berlin Institute of Technology, Department of Biotechnology, Chair of Brewing Science, Seestraße 13, 13353 Berlin, Germany |
| |
Abstract: | In food, the mycotoxin deoxynivalenol (DON) often occurs in conjunction with its 3-β-d-glucopyranoside (D3G). The transformation of DON to D3G through glucosylation is catalysed by plant enzymes, however, the exact circumstances are not well understood. In order to investigate the role of enzymatic glucosylation in germinating grains, DON treated kernels were steeped and germinated under laboratory conditions. Furthermore, the effect of malting on the DON content of the contaminated barley was investigated. In all cases, DON and its derivatives were quantified by HPLC-MS/MS before, during and after the experiments. Amongst the six tested cereals; wheat, rye, barley, spelt, and millet transformed DON to D3G during germination whilst the oats were inactive. For wheat, barley, and spelt the initial DON content was reduced by 50%, with the loss being almost entirely accounted for by D3G formation. As D3G might be cleaved during digestion, the elevated D3G concentration may obscure the toxicologically relevant DON content in processed food and beer. The germination process has a major influence on the “masking” of DON, leading to high quantities of D3G that may be missed in common mycotoxin analyses. |
| |
Keywords: | 15-AcDON 15-acetyldeoxynivalenol 3-AcDON 3-acetyldeoxynivalenol D3G d-glucopyranoside" target="_blank">DON-3-β-d-glucopyranoside DON deoxynivalenol ESI electrospray ionisation HPLC high-performance liquid chromatography LOD limit of detection LOQ limit of quantification MS mass spectrometry MS/MS tandem mass spectrometry |
本文献已被 ScienceDirect 等数据库收录! |
|