An improved method for thin layer chromatographic analysis of saponins |
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| Authors: | Om P. Sharma Neeraj KumarBikram Singh Tej K. Bhat |
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| Affiliation: | a Biochemistry Laboratory, Indian Veterinary Research Institute, Regional Station, Palampur 176 061, Himachal Pradesh, India
b Division of Natural Plant Products, Institute of Himalayan Bioresource Technology, Palampur 176 061, Himachal Pradesh, India |
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| Abstract: | Analysis of saponins by thin layer chromatography (TLC) is reported. The solvent system was n-butanol:water:acetic acid (84:14:7). Detection of saponins on the TLC plates after development and air-drying was done by immersion in a suspension of sheep erythrocytes, followed by washing off the excess blood on the plate surface. Saponins appeared as white spots against a pink background. The protocol provided specific detection of saponins in the saponins enriched extracts from Aesculusindica (Wall. ex Camb.) Hook.f., Lonicera japonica Thunb., Silene inflata Sm., Sapindusmukorossi Gaertn., Chlorophytum borivilianum Santapau & Fernandes, Asparagusadscendens Roxb., Asparagus racemosus Willd., Agave americana L., Camellia sinensis [L.] O. Kuntze. The protocol is convenient, inexpensive, does not require any corrosive chemicals and provides specific detection of saponins. |
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| Keywords: | Thin layer chromatography Saponins Haemolysis |
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