Antiatherogenic effect of guava leaf extracts inhibiting leucocyte-type 12-lipoxygenase activity |
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Authors: | Yuki KawakamiTomoko Hosokawa Tomoko MorinakaShioka Irino Shiori HiranoHirokazu Kobayashi Akiko YoshiokaToshiko Suzuki-Yamamoto Miyuki YokoroMasumi Kimoto Hideaki TsujiHiromi Yamashita Shinobu DoiChikao Yutani Rina KatoHiroyuki Itabe Teruyuki KanadaTakahiko Hada Yoshitaka Takahashi |
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Affiliation: | a Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan b Department of Life Science, Faculty of Science, Okayama University of Science, 1-1 Ridai-cho, Okayama 700-0005, Japan c Department of Biological Chemistry, Showa University School of Pharmacy, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan d Research Center, Bizen Chemical Co., Ltd., 363 Tokutomi, Akaiwa, Okayama 709-0716, Japan |
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Abstract: | Oxidative modification of low density lipoprotein (LDL) is one of the critical steps for the development of atherosclerosis and leucocyte-type 12-lipoxygenase, highly expressed in macrophages, has been suggested to play an essential role in this process. In the present study, we show that guava leaf extracts inhibited, not only the leucocyte-type 12-lipoxygenase activity, but also LDL oxidation, mediated by the enzyme-overexpressing macrophage-like J774A.1 cells. Oral administration of guava leaf extracts to apoE-knockout mice at 100 mg of dry extracts/kg of body weight, once a day for 16 weeks, significantly reduced the area of atherogenic lesions developed in the aorta and aortic sinus. The major components inhibiting leucocyte-type 12-lipoxygenase contained in guava leaf extracts were identified as ethyl gallate and quercetin. The inhibitory effects of guava leaf extracts on the leucocyte-type 12-lipoxygenase activity, as well as on cell-mediated LDL oxidation, might be involved in the antiatherogenic effect of the extracts. |
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Keywords: | Leucocyte-type 12-lipoxygenase Arachidonic acid LDL oxidation Macrophages Psidium guajava Atherosclerosis |
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