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Anti-allergic activity of grapeseed extract (GSE) on RBL-2H3 mast cells |
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| Authors: | Bing-Hung Chen Mei-Huei HungJeff Yi-Fu Chen Hsueh-Wei ChangMeng-Lung Yu Lei WanFuu Jen Tsai Tzu-Pin WangTzu-Fun Fu Chien-Chih Chiu |
| Affiliation: | a Department of Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan b Department of Biomedical Science and Environmental Biology, Graduate Institute of Natural Products, Center of Excellence for Environmental Medicine, Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan c Department of Medical Genetics and Medical Research, Graduate Institute of Chinese Medical Science, China Medical University Hospital, Taichung, Taiwan d Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan e Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, Tainan, Taiwan |
| Abstract: | Grapeseed extract (GSE) is a rich source of natural phenolic compounds and possesses various pharmacological activities, including antioxidant, anticarcinogenic and anti-inflammatory properties. However, effects of GSE on immunoglobulin (Ig) E-mediated allergic responses still remain elusive. In the present study, the effects of GSE on activation and degranulation of RBL-2H3 mast cells were investigated. GSE pretreatments (20-100 μg/ml) reduced IgE-antigen mediated release of β-hexosaminidase and histamine in RBL-2H3 cells. Additionally, GSE reversibly inhibited expression of FcεRI on RBL-2H3 cells. GSE treatments caused a significant elevation of intracellular cAMP levels, whereas the Ca2+ influx upon antigen stimulation was inhibited. Suppression on FcεRI expression together with decreased calcium uptake and increased cAMP level might be involved in attenuated degranulation of mast cells by GSE treatment. Our results suggest a possible pharmaceutical application of GSE in treating type I allergic diseases. |
| Keywords: | BSA bovine serum albumin cAMP cyclic adenosine monophosphate DNP dinitrophenyl FcεRI high-affinity IgE receptor FITC fluorescein isothiocyanate IgE immunoglobulin E IL interleukin mIgE-DNP murine anti-dinitrophenol IgE |
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