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Microbial transformation of tectoridin from Pueraria flos by Lactobacillus and bifidobacteria |
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| Authors: | Ying LiuGang Cheng Ting HanHong Yang SA IbrahimWen Huang |
| Affiliation: | a College of Food Science and Technology, Huazhong Agriculture University, Wuhan, Hubei 430070, China b Key Lab for Biotechnology of National Commission for Nationalities, College of Life Science, South Central University for Nationalities, Wuhan, Hubei 430078, China c Department of Family and Consumer Sciences, North Carolina A&T State University, 171 Carver Hall, Greensboro, NC 27411, United States |
| Abstract: | Tectoridin could be hydrolyzed to tectorigenin by β-glucosidase-producing intestinal bacteria. In this study, nine strains of Lactobacillus and bifidobacteria were screened for high levels of β-glucosidase activity. We investigated their ability to transform tectoridin from Pueraria flos to tectorigenin. Lactobacillus reuteri DSM20016 showed the highest cell-envelope associated β-glucosidase activity, whereas the intracellular β-glucosidase activity from Bifidobacterium adolescentis ATCC15703 was higher than the other screened bacterial strains. L. reuteri DSM20016, Lactobacillus rhamnosus GGB41031 and B. adolescentis ATCC15703 showed high bioconversion rate of tectoridin. L. reuteri DSM20016 showed the highest bioconversion efficiency of tectoridin, 100% tectoridin was hydrolyzed and there was an approximate 185-fold increase in the concentration of tectorigenin after 24 h. The present study suggests that L. reuteri DSM20016, L. rhamnosus GGB41031 and B. adolescentis ATCC15703 have great potential for converting tectoridin from Pueraria flos to more bioactive tectorigenin. |
| Keywords: | Tectoridin Tectorigenin Lactobacillus Bifidobacteria β-Glucosidase activity Bioconversion |
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